| CAS No.: | 67727-97-3 |
|---|---|
| Formula: | C187h291n45o59 |
| EINECS: | N/a |
| Type: | Peptides Powder |
| Appearance: | Powder |
| Quality: | Top Quality |
| Samples: |
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| Customization: |
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| Product name | Pnc 27 |
| Appearance | White powder |
| Purity | 99% |
| Storage | Keep in a cool, dry, dark location in a tightly sealed container or cylinder. |
| Shelf Life | 24 Months |
Description
The anticancer peptide PNC-27, which contains an HDM-2-binding domain corresponding to residues 12-26 of
p53 and a transmembrane-penetrating domain, has been found to kill cancer cells (but not normal cells) by
inducing membranolysis. We find that our previously determined 3D structure of the p53 residues of PNC-27 is
directly superimposable on the structure for the same residues bound to HDM-2, suggesting that the peptide may
target HDM-2 in the membranes of cancer cells.
Application&Function
We now find significant levels of HDM-2 in the membranes of a varietyof cancer cells but not in the membranes of
several untransformed cell lines. In colocalization experiments, we find that PNC-27 binds to cell membrane-bound
HDM-2. We further transfected a plasmid expressing full-length HDM-2 with a membrane-localization signal into
untransformed MCF-10-2A cells not susceptible to PNC-27 and found that these cells expressing full-length HDM-2
on their cell surface became susceptible to PNC-27. We conclude that PNC-27 targets HDM-2 in the membranes of
cancer cells, allowing it to induce membranolysis of these cells selectively.
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